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1 g of the substance to be examined and 80 mg of the reference substance in 10 ml of water R, add 2 ml of dilute sodium hydroxide solution R and shake with 2 quantities, each of 20 ml, of methylene chloride R; combine the organic layers, wash with water R, dry over anhydrous sodium sulphate R, evaporate to dryness and dissolve the residues separately, each in 2 ml of methylene chloride R.
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Use for the test not fewer than 15 fattening pigs of the age to be recommended and that do not have antibodies against Aujeszkyвs disease virus or against a fraction of the virus. OS. Water (see Tests).